ERC SUMMIT – Site-specific Ultrasensitive Magnetic resonance of Mixtures for Isotopic Tracking

Exploring the potential of fast and hyperpolarized nuclear magnetic resonance methods for “omics” sciences, this project is led by Patrick Giraudeau and the MIMM research team relies on cutting-edge equipments from the CEISAM NMR platform.

5-year project

2019 - 2024

€ 1 999 768

ERC funds

211

person-months effort over the 5 years

7

internal team members

summit erc logo ceisam.png

The SUMMIT team is developing new tools based on nuclear magnetic resonance (NMR) to obtain information on complex biological questions in the areas of life, environmental, food and forensic sciences.

NMR is a cutting-edge technique that provides crucial information on molecules in biological samples at the atomic level. For instance, NMR can help finding new biomarkers of a certain pathology, or provide information on the authenticity of a food sample. In this context, SUMMIT is developing more sensitive and resolutive methods, that will be applied to a variety of biological questions.

 

In more details

Metabolomics, fluxomics and isotopomics show great promises towards tracking the origin of biomarkers in complex biological environments, in the areas of life, environmental, food and forensic sciences. NMR spectroscopy is a major cutting-edge analytical tool for such “omics” sciences thanks to its high quantitative and structure elucidation potential, and to its ability to access site-specific isotope content. 13C NMR plays a central role, but numerous applications are currently out of reach of 13C NMR due to its low sensitivity.

The SUMMIT team is developing a groundbreaking analytical workflow relying on two of the most powerful NMR methods: dissolution dynamic nuclear polarization and ultrafast 2D NMR. This approach will allow the simultaneous measurement of 13C fingerprints from multiple low-concentrated biomarkers molecules in complex mixtures, which is impossible with existing methods. The high potential of this analytical strategy will be demonstrated on a variety of relevant biological studies, through applications in metabolomics, fluxomics and isotopomics.

Project team

Patrick GIRAUDEAU

Professor at the University of Nantes

Project leader

Hélène BONIN

Project Manager

Finances, human ressources, management and reporting

Clément PRAUD

PhD student
NMR pulse sequence developments
(2020-2023)

Marine PIOU

Engineer
Signal processing
(2021-2022)

Jean-Nicolas DUMEZ

CNRS research associate

NMR pulse sequence developments

Arnab DEY

Post-doctoral research associate
d-DNP developments
(2019-2022)

Marine LETERTRE

Post-doctoral research associate
NMR metabolomics
(2021-2023)

Benoît CHARRIER

Engineer

d-DNP instrumentation

Célia LHOSTE

PhD Student
NMR pulse sequence developments
(2020-2023)

Victor RIBAY

PhD student
Development in d-DNP and metabolomics
(2021-2024)

Publications

6.Ultrafast 2D NMR for the analysis of complex mixtures (link)
Célia Lhoste, Benjamin Lorandel, Clément Praud, Achille Marchand, Rituraj Mishra, Arnab Dey, Aurélie Bernard, Jean-Nicolas Dumez, Patrick Giraudeau

This review article describes ultrafast 2D NMR and its applications in the fields of monitoring, metabolomics, hyperpolarization and oriented media.

 

5.Nuclear Magnetic Resonance Spectroscopy in Clinical Metabolomics and Personalized Medicine: Current Challenges and Perspectives (link)
Marine P. M. Letertre, Patrick Giraudeau and Pascal de Tullio

 

4.Ultrafast 2D 1H-1H NMR spectroscopy of DNP-hyperpolarised substrates for the analysis of mixtures (link)
Kawarpal Singh, Corentin Jacquemmoz, Patrick Giraudeau, Lucio Frydman, Jean-Nicolas Dumez

 

3.Combined Nuclear Magnetic Resonance Spectroscopy and Mass Spectrometry Approaches for Metabolomics (link)
Marine P. M. Letertre, Gaud Dervilly, and Patrick Giraudeau

Graphical abstract

 

2.Hyperpolarized NMR Metabolomics at Natural 13C Abundance (link)
Arnab Dey, Benoît Charrier, Estelle Martineau, Catherine Deborde, Elodie Gandriau, Annick Moing, Daniel Jacob, Dmitry Eshchenko, Marc Schnell, Roberto Melzi, Dennis Kurzbach, Morgan Ceillier, Quentin Chappuis, Samuel F. Cousin, James G. Kempf, Sami Jannin, Jean-Nicolas Dumez, and Patrick Giraudeau

TOC_030620

 

1.NMR-based metabolomics and fluxomics: developments and future prospects (link)
Patrick GIRAUDEAU

NMR-based metabolomics and fluxomics developments and future prospects