ERC SUMMIT – Site-specific Ultrasensitive Magnetic resonance of Mixtures for Isotopic Tracking
2019 - 2024
€ 1 999 768
person-months effort over the 5 years
internal team members
The SUMMIT team is developing new tools based on nuclear magnetic resonance (NMR) to obtain information on complex biological questions in the areas of life, environmental, food and forensic sciences.
NMR is a cutting-edge technique that provides crucial information on molecules in biological samples at the atomic level. For instance, NMR can help finding new biomarkers of a certain pathology, or provide information on the authenticity of a food sample. In this context, SUMMIT is developing more sensitive and resolutive methods, that will be applied to a variety of biological questions.
In more details
Metabolomics, fluxomics and isotopomics show great promises towards tracking the origin of biomarkers in complex biological environments, in the areas of life, environmental, food and forensic sciences. NMR spectroscopy is a major cutting-edge analytical tool for such “omics” sciences thanks to its high quantitative and structure elucidation potential, and to its ability to access site-specific isotope content. 13C NMR plays a central role, but numerous applications are currently out of reach of 13C NMR due to its low sensitivity.
The SUMMIT team is developing a groundbreaking analytical workflow relying on two of the most powerful NMR methods: dissolution dynamic nuclear polarization and ultrafast 2D NMR. This approach will allow the simultaneous measurement of 13C fingerprints from multiple low-concentrated biomarkers molecules in complex mixtures, which is impossible with existing methods. The high potential of this analytical strategy will be demonstrated on a variety of relevant biological studies, through applications in metabolomics, fluxomics and isotopomics.
Authors: Célia Lhoste, Margherita Bazzoni, Justine Bonnet, Aurélie Bernard, François-Xavier Felpin, Patrick Giraudeau, Jean-Nicolas Dumez
15. Scaling-up metabolomics: Current state and perspectives (link)
Authors: Ghina Hajjar, Millena C. Barros Santos, Justine Bertrand-Michel, Cécile Canlet, Florence Castelli, Nicolas Creusot, Sylvain Dechaumet, Binta Diémé, Franck Giacomoni, Patrick Giraudeau,Yann Guitton, Etienne Thévenot, Marie Tremblay-Franco, Christophe Junot, Fabien Jourdan, François Fenaille, Blandine Comte, Pierre Pétriacq, Estelle Pujos-Guillot
14. NMR metabolite quantification of a synthetic urine sample: an inter-laboratory comparison of processing workflows (link)
Authors: Cécile Canlet, Catherine Deborde, Edern Cahoreau, Grégory Da Costa, Roselyne Gautier, Daniel Jacob, Cyril Jousse, Mélia Lacaze, Inès Le Mao,
Estelle Martineau, Lindsay Peyriga, Tristan Richard, Virginie Silvestre, Mounir Traïkia, Annick Moing, Patrick Giraudeau
13. Book chapter: Fast 2D NMR for metabolomics (link)
Authors: Clément Praud, Marine P.M. Letertre, Arnab Dey, Jean-Nicolas Dumez, Patrick Giraudeau
12. Book chapter: Fast 2D NMR for reaction and process monitoring (link)
Authors: Margherita Bazzoni, Benjamin Lorandel, Célia Lhoste, Patrick Giraudeau, Jean-Nicolas Dumez
11. Hyperpolarized 13C NMR Spectroscopy of Urine Samples at Natural Abundance by Quantitative Dissolution Dynamic Nuclear Polarization (link)
Authors: Victor Ribay, Arnab Dey, Benoît Charrier, Clément Praud, Joris Mandral, Jean-Nicolas Dumez, Marine P.M. Letertre, and Patrick Giraudeau
10. Quantitative NMR spectroscopy of complex mixtures (link)
Author: Patrick Giraudeau
9. Hyperpolarized NMR metabolomics (link)
Authors: Victor Ribay, Clément Praud, Marine P. M. Letertre, Jean-Nicolas Dumez and Patrick Giraudeau
8. In-line Multidimensional NMR Monitoring of Photochemical Flow Reactions (link)
Authors: Margherita Bazzoni, Célia Lhoste, Justine Bonnet, Kouakou Eric Konan, Aurélie Bernard, Patrick Giraudeau, François-Xavier Felpin,
and Jean-Nicolas Dumez
This work demonstrates the in-line monitoring of a flow photochemical reaction using 1D and ultrafast 2D NMR methods at high magnetic field.
7. Fine optimization of a dissolution dynamic nuclear polarization experimental setting for 13C NMR of metabolic samples (link)
Authors: Arnab Dey, Benoît Charrier, Karine Lemaitre, Victor Ribay, Dmitry Eshchenko, Marc Schnell, Roberto Melzi, Quentin Stern,
Samuel F. Cousin, James G. Kempf, Sami Jannin, Jean-Nicolas Dumez, and Patrick Giraudeau
6. Ultrafast 2D NMR for the analysis of complex mixtures (link)
Authors: Célia Lhoste, Benjamin Lorandel, Clément Praud, Achille Marchand, Rituraj Mishra, Arnab Dey, Aurélie Bernard, Jean-Nicolas Dumez, Patrick Giraudeau
This review article describes ultrafast 2D NMR and its applications in the fields of monitoring, metabolomics, hyperpolarization and oriented media.
5. Nuclear Magnetic Resonance Spectroscopy in Clinical Metabolomics and Personalized Medicine: Current Challenges and Perspectives (link)
Authors: Marine P. M. Letertre, Patrick Giraudeau and Pascal de Tullio
4. Ultrafast 2D 1H-1H NMR spectroscopy of DNP-hyperpolarised substrates for the analysis of mixtures (link)
Authors: Kawarpal Singh, Corentin Jacquemmoz, Patrick Giraudeau, Lucio Frydman, Jean-Nicolas Dumez
3. Combined Nuclear Magnetic Resonance Spectroscopy and Mass Spectrometry Approaches for Metabolomics (link)
Authors: Marine P. M. Letertre, Gaud Dervilly, and Patrick Giraudeau
2. Hyperpolarized NMR Metabolomics at Natural 13C Abundance (link)
Authors: Arnab Dey, Benoît Charrier, Estelle Martineau, Catherine Deborde, Elodie Gandriau, Annick Moing, Daniel Jacob, Dmitry Eshchenko, Marc Schnell,
Roberto Melzi, Dennis Kurzbach, Morgan Ceillier, Quentin Chappuis, Samuel F. Cousin, James G. Kempf, Sami Jannin, Jean-Nicolas Dumez, and Patrick Giraudeau
1. NMR-based metabolomics and fluxomics: developments and future prospects (link)
Author: Patrick GIRAUDEAU