Analytical equipment

La plateforme SMRI comprend actuellement deux équipements (Thermo Electron et Sercon) avec trois configurations possibles :

Couplage Analyseur Élémentaire / SMRI qui permet l’analyses 13C, 15N, 18O et 34S des échantillons :

  • AE/ SMRI Integra 2 de marque Sercon, analyse 13C, 15N et 18O
  • AE/ SMRI Delta V Advantge Thermo Electron, analyse 13C et 15N simultanément, 18O et 34S

 

Couplage Chromatographie Gazeuse / interface Combustion / SMRI qui permet l’analyse de mélanges de molécules :

  • CG / C/ SMRI Delta V Advantge Thermo Electron qui permet l’analyse de chaque molécules du mélange, mesure 13C et 15N

photo_smri

Staff:

SCHIPHORST Anne-Marie
Office 043
Phone: 02 51 12 57 05
or 02 76 64 50 84
Link

Elemental analysis

Elemental analyzer for quantitative dosage of C, H, N and S elements (Flash 2000 de Thermo Scientific)

 

Flash 2000 apparatus from ThermoFisher Scientific for the simultaneous analysis of carbon, nitrogen, oxygen and sulfur in 1 mg of solid or liquid sample. Sample are analysed two times.

ceisam flash 2000 ThermoFisher Scientific

Principle

The sample (1 mg – microgramme precision) is pyrolize under oxygen at 960°C.

Nitrogen, carbon, hydrogen and sulphur are, by a succession of reactions, oxidized in carbon dioxide, water, dinitrogen and sulphur dioxide. These gases are then separated by column chromatography, detected by a cathetometer and quantified by integration of the resulting peak from the conduction variation of the so-called cathetometer.

The viability of the result is established at the end of the analysis by a standard.

On stable and clean products, the percentage given by the analyser is more or less 0.4% of the calculated pourcentages. (absolute accuracy = 0.2 %).

Staff:

Denis LOQUET
Office 123
Phone: +332 51 12 57 13
Link

Julie HEMEZ
Office 042
Phone: +332 76 64 51 72
Link

Method: Particle size analysis using dynamic light scattering (DLS) (CPER CISPEO)

Device: Cordouan VascoKin™

170° back-scattering working mode, volume range 50 µL-4 mL

Accessories:

  • 659 nm excitation laser diode head
  • APD detection (dead time 35 ns, pulse width 14 ns, afterpulse < 1%)
  • Tête de mesure déportée
  • Gamme de tailles : 0,5 nm à 10 um
  • Concentration : 1.10-5 à 40 % massique
  • Résolution temporelle : 200 ms

Cordouan VascoKin

Atomic Absorption Spectroscopy

absorption_atomique

Atomic Absorption Spectrometer THERMO SCIENTIFIC ICE 3300

Flame "Air/Acetylene" or "Nitrous Oxyde/Acetylene"

Analysis with Flame Air/Acetylene:
Li, Na, Mg, K, Ca, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, Ge, Rb, Sr, Ru, Rh, Pd, Ag, Cd, In, Sn, Sb, Te, Cs, Ba, Ir, Pt, Au, Hg, Tl, Pb, Bi.
Available lamps in the lab : Pb, Fe/Ni, Na/K, Ca/Si, Cd/Zn, Mg, Ag, Ga, Sn, Cu and Pd.

Analysis with Flame Nitrous oxide /Acetylene :
Be, B, Al, Si, P, Ca, Sc, Ti, V, Cr, Ga, Ge, As, Se, Sr, Y, Zr, Nb, Mo, Rh, Sn, Ba, La, Hf, Ta, W, Re, Os, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, Lu, Th, U.
Available lamps in the lab : Ca/Si, Ga, Sn, Gd, As, P, Ho, Ti, Zr and Pt.

 

Staff:

Nathalie GUICHARD
Office 240
Phone: +332 51 12 53 28
Link

François-Xavier LEFEVRE
Office 240
Phone: +332 51 12 54 05
Link

 

Atomic force microscope

Operation CPER "CEISAM Atomic Force Microscope" of the CISPEO project is cofunded by the European Union through the funds of the region Pays de la Loire (FEDER) .

ceisam europe region feder

ATOMIC FORCE MICROSCOPE JPK NanoWizard®4 combined with NIKON Ti2-U inverted optical microscope

Measuring mode :

  • Contact.
  • Dynamic.
  • Force Modulation (QI Advanced TM mode).
  • Kelvin Probe Force Microscopy (KPM mode).

 

Sample holder :

  • Temperature module (-30°C to +120°C +/- 0.1°C) designed for opaque samples (JPK HCM TM ). Sample size : 15x15 mm. .
  • Temperature module (RT to +60°C +/- 0.1°C) designed for transparent samples (PetriDishHeater TM ). Compatible with 35 mm Petri dishes.

 

Inverted Microscope :

Objective:

  • Nikon CFI S P-Fluor ELWD 60xC/ 0.95/WD : 0,21-0,11mm.
  • Nikon CFI S P-Fluor ELWD 40xC/ 0.95/ WD : 0,25-0,16mm.
  • Nikon CFI Plan Apochromat Lambda 20X/ 0.75/ 1,00 mm.

LED Source.

Fluorescence filters :

  • DAPI-50LP-A.
  • FITC-3540C.
  • Cy3-4040C.
  • Cy5-4040C.

 

Isolation:

JPK acoustic enclosure with Accurion's Halcyonics i4 acitve vibration isolation system.

  • Cy3-4040C.
  • Cy5-4040C.

operation

Le microscope à Force Atomique est adapté aux échantillons inorganiques - organiques - biologiques – sous air ou en milieu liquide.
Les mesures sont réalisées par François-Xavier LEFEVRE.

Staff

François-Xavier LEFEVRE
Office 240
Phone: +332 51 12 54 05
Link