Vincent PORTALURI, PhD student in CEISAM MIMM team, will defend her thesis entitled “Development of new applications in the analysis of food authenticity by Nuclear Magnetic Resonance (NMR)”on October 12th 2021 at 14h in CEISAM’s conference room “Marie Curie”.
Food authentication offers product traceability to consumers, while acting against frauds. Nuclear Magnetic Resonance (NMR) is a premium analytical tool for both position-specific isotope analysis (PSIA) and spectral fingerprinting. Several targets were set for this work: increase 13C-isotope ratio monitoring-NMR sensitivity through methodological advances, and develop a solution for 1H NMR Profiling data curation. The first part aimed at implementing routinely adiabatic refocused INEPT (Insensitive Nuclei Enhanced by Polarization Transfer) pulse sequence to get the full 13C isotopic profile of several molecules such as ethanol and vanillin. Great analytical performances were obtained and the definition of an empirical correction allowed the use of historical databases. This work was described in two publications. Secondly, an automated tool was developed to exploit data generated by non-targeted 1H NMR honey analysis. Pertinent information was synthesized in order to offer a clear interpretation of the experiment from an authentication point of view, including botanical and geographical origin verification and addition of exogenous sugars. All developed methods were transferred and applied routinely. Concepts explored during these projects paved the way for other matrices control, in order to extend the scope of NMR methods (either in isotope or metabolomic field) for food authentication.
Food authentication; 13C-irm-NMR; Chemometrics; Vanillin; Honey.