Bioorthogonal ligation methods are extensively explored for the development of protein conjugates, which are of considerable importance in the therapeutic field and in biotechnology industries. The electrochemically promoted tyrosine-click-chemistry for protein labelling (e-CLICK) project will considerably broaden the scope of the click-Y by providing the reactive PTAD specie on demand, in different buffers, and without the needs of an oxidizing chemical. This ambitious goal will be reached by using a more promising strategy in terms of chemo-selectivity and protein modification. We propose here to develop the first electrochemically driven probes for Y-specific protein conjugations. The application of an appropriate electrochemical potential will allow us to activate the PTAD dormant specie in situ, on demand, without oxidizing the sensitive amino-acids from the protein or the ligands linked to the PTAD.

Mohammed BOUJTITA

Associate professor

Project Manager

UGSF

Structural and Functional Glycobiology Unit, Physiology of Nutritional Adaptations