ERC SUMMIT – Site-specific Ultrasensitive Magnetic resonance of Mixtures for Isotopic Tracking
2019 - 2024
€ 1 999 768
person-months effort over the 5 years
internal team members
The SUMMIT team is developing new tools based on nuclear magnetic resonance (NMR) to obtain information on complex biological questions in the areas of life, environmental, food and forensic sciences.
NMR is a cutting-edge technique that provides crucial information on molecules in biological samples at the atomic level. For instance, NMR can help finding new biomarkers of a certain pathology, or provide information on the authenticity of a food sample. In this context, SUMMIT is developing more sensitive and resolutive methods, that will be applied to a variety of biological questions.
In more details
Metabolomics, fluxomics and isotopomics show great promises towards tracking the origin of biomarkers in complex biological environments, in the areas of life, environmental, food and forensic sciences. NMR spectroscopy is a major cutting-edge analytical tool for such “omics” sciences thanks to its high quantitative and structure elucidation potential, and to its ability to access site-specific isotope content. 13C NMR plays a central role, but numerous applications are currently out of reach of 13C NMR due to its low sensitivity.
The SUMMIT team is developing a groundbreaking analytical workflow relying on two of the most powerful NMR methods: dissolution dynamic nuclear polarization and ultrafast 2D NMR. This approach will allow the simultaneous measurement of 13C fingerprints from multiple low-concentrated biomarkers molecules in complex mixtures, which is impossible with existing methods. The high potential of this analytical strategy will be demonstrated on a variety of relevant biological studies, through applications in metabolomics, fluxomics and isotopomics.
Ultrafast 2D 1H-1H NMR spectroscopy of DNP-hyperpolarised substrates for the analysis of mixtures (link)
Kawarpal Singh, Corentin Jacquemmoz, Patrick Giraudeau, Lucio Frydman, Jean-Nicolas Dumez
We show that TOCSY and multiple-quantum (MQ) 2D NMR spectra can be obtained for mixtures of substrates hyperpolarised by dissolution dynamic nuclear polarisation (D-DNP).
Combined Nuclear Magnetic Resonance Spectroscopy and Mass Spectrometry Approaches for Metabolomics (link)
Marine P. M. Letertre, Gaud Dervilly, and Patrick Giraudeau*
Hyperpolarized NMR Metabolomics at Natural 13C Abundance (link)
Arnab Dey, Benoît Charrier, Estelle Martineau, Catherine Deborde, Elodie Gandriau, Annick Moing, Daniel Jacob, Dmitry Eshchenko, Marc Schnell, Roberto Melzi, Dennis Kurzbach, Morgan Ceillier, Quentin Chappuis, Samuel F. Cousin, James G. Kempf, Sami Jannin, Jean-Nicolas Dumez, and Patrick Giraudeau*
NMR-based metabolomics and fluxomics: developments and future prospects (link)
This review article describes how some of the most recent developments emerging from the NMR community could act as game changers for metabolomics and fluxomics in the near future.